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KMID : 0350519960490020573
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.573 ~ p.582
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In Vitro Immune Stimulation of Murine Splenocytes and Peritoneal Macrophages Induced by Silicone Gel
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Abstract
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A serious concern has been given to the possible cause of autoimmune disease when silicone gel implant was used in breast surgery. The silicone gel has recently been suspected of being immunogenic. The action of silicone gel against the immune
system
has not been understood.
This experiment was performed to test the silicone gel for evoking immune response in vitro condition by 1) assessing the effect of silicone gel on the T-dependent antibody response and B cell activation response in splenocytes by plaque forming
cell
assay and suspension hemolytic assay, and 2) determining whether silicone gel is capable of enhancing lymphocyte proliteration in the presence of mitogens, such as lipopolysaccharide, concanavalin A, phytohemagglutinin-P and pokeweed mitogen, as
well as
mixed lymphocyte response, or not, and 3) testing the cytotoxicity of peritoneal marophages under the influence of silicone gel. The immunocytes for experiment were obtained from the splenocytes and peritoneal macrophages of ICR, BDF1, and B6C3F1
mice.
@ES The results were as follows;
@EN 1. A significant increase in T-dependent antibody response by silicone gel was observed (P<0.01). There was an increase in B cell activation response by silicone gel, but it was not statistically significant.
2. No significant difference was observed in mixed lymphocyte response to the presence or absence of silicone gel.
3. Silicone gel was capable of enhancing lymphocyte proliferation in the presence of PHA-P (P<0.01), but not in the presence of Con A, LPS and PWM.
4. There was a significant stimulation effect of silicone gel on cytotoxicity of macrophage against YAC-1 target cells (P<0.01).
These results suggest that silicone gel has a stimulation effect on T-dependent antibody response in an in vitro condition due to activation of macrophage rather than direct action on T and/or B cell. Thus there is a possibility that silicone gel
could
mediate an autoimmune disease.
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